FIG 7.

RO3306 treatment reduces lethality in JEV-infected WT mice. (A to H) WT or MAVS^−/− mice were infected intraperitoneally with 1 × 10 ⁴ PFU of the JEV virus or the same volume of DMEM. RO3306 (10 mg/kg body weight) or an equal volume of DMSO was intravenously administered on day 3 postinfection. Mice were sacrificed on days 3 (before intravenously administered RO3306) and 4 postinfection, and serum and brain samples were collected for the subsequent analyses. Mice survival rate (A and E) and clinical signs of the disease (B and F) in each experimental group were evaluated for 21 days postinfection (n = 10). Viral titers in serum (C and G) and brain samples (D and H), harvested on days 3 and 4 postinfection, were measured by plaque assay (n = 5). All data were pooled from three independent experiments. *, P < 0.05; ***, P < 0.001. ns, nonsignificant.