TABLE 1.
Primers used in this studya
| Primer | Sequence |
|---|---|
| JEV-NS1 and NS1′-F | CGGGATCCGACACTGGATGTGCCATTGACAT |
| JEV-NS1′52aa-F | CGGGATCCTTTAATGGTGAAATGGTTGAC |
| JEV-NS1-R | CCCTCGAGTCAAGCATCAACCTGTGATCTAACGA |
| JEV-NS1′-R | CCCTCGAGTCAGTGTAAGTGATGCCCCCAAGCAT |
| hCDK1-F | CCAAGCTTGCCACCATGGAAGATTATACCAAAATAGAGAAAATT |
| hCDK1-R | CGCGGATCCGCG TCA CATCTTCTTAATCTGATT |
| hCDC25C-F | CCAAGCTT GCCACC ATGTCTACGGAACTCTTCTCATC |
| hCDC25C-R | CGCGGATCC TCA TCATGGGCTCATGTCCTTCACC |
| qRT-PCR | |
| miR-22 stem-loop | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACAGTT |
| miR-22-F | AAGCTGCCAGTTGAAGAACTGT |
| Universal reverse | GTGCAGGGTCCGAGGT |
| hIFN-β-F | TGCTCTGGCACAACAGGTAG |
| hIFN-β-R | AGCCTCCCATTCAATTGCCA |
| hβ-actin-F | AGCGGGAAATCGTGCGTGAC |
| hβ-actin-R | GGAAGGAAGGCTGGAAGAGTG |
| hMAVS-F | CCGTTTGCTGAAGACAAG |
| hMAVS-R | GCTCTGGTAGACAGAGGC |
| JEV-F | TGGTTTCATGACCTCGCTCTC |
| JEV-R | CCATGAGGAGTTCTCTGTTTCT |
| JEV-probe | FAM-CCTGGACGCCCCCTTCGAGCACAGCGT-TAMRA |
| RNA oligonucleotides | |
| miR-22 inhibitor control | ACAGUUCUUCAACUGGCAGCUU |
| Inhibitor | CAGUACUUUUGUGUAGUACAA |
a
h, human; F, forward; R, reverse.