TABLE 2.
Biofilm-forming capacity, curli and cellulose expression, motility, and the presence of virulence genes and the locus of heat resistance in isolates of top seven E. coli strains (n = 745) collected from cattlea
| Cellulose, curli, and virulence attribute | No. of isolates with biofilm-forming capacity (no. with LHR)b |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Non-biofilm former |
Weak |
Moderate |
Strong |
Extreme |
||||||
| stx1 negative | stx1 positive | stx1 negative | stx1 positive | stx1 negative | stx1 positive | stx1 negative | stx1 positive | stx1 negative | stx1 positive | |
| Not CCMc | ||||||||||
| stx2 negative | ||||||||||
| eae negative | 72 (4 LHR) | 32 (1 LHR) | 4 | 3 | 3 (1 LHR) | 5 (2 LHR) | 1 | 1 LHR | 0 | 0 |
| eae positive | 94 (1 LHR) | 116 | 1 | 3 | 1 | 2 (1 LHR) | 0 | 4 (1 LHR) | 0 | 0 |
| stx2 positive | ||||||||||
| eae negative | 5 (3 LHR) | 11 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
| eae positive | 22 (2 LHR) | 173 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 1 |
| CCMd | ||||||||||
| stx2 negative | ||||||||||
| eae negative | 53 | 20 | 3 | 1 | 2 | 2 | 1 | 1 | 1 | 0 |
| eae positive | 24 | 35 | 1 | 1 | 1 | 2 | 1 | 0 | 0 | 0 |
| stx2 positive | ||||||||||
| eae negative | 1 | 8 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
| eae positive | 14 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Total | 285 | 407 | 9 | 10 | 9 | 13 | 3 | 7 | 1 | 1 |
Shiga toxin-producing E. coli isolates are shaded in gray. Enterohemorrhagic E. coli isolates are in boldface type. LHR, locus of heat resistance.
Biofilm-forming capacity is as follows: non-biofilm former, x < ODc; weak, ODc < x < 2× ODc; moderate, 2× ODc < x < 4× ODc; strong, 4× ODc < x < 8× ODc; extreme, 16× ODc < x. No isolates were classified as very strong biofilm formers (8× ODc < x < 16× ODc).
Not CCM, the isolate does not have all three of the following characteristics: motility, expression of curli, and expression of cellulose.
CCM, the isolate is motile and expresses both curli and cellulose.