Ten additional L. monocytogenes strains, encompassing an array of serotypes and lineages, all have enhanced root colonization when cocultured with ES620 (P. simiae). L. monocytogenes strains were coinoculated with ES620 for 24 h at RT. After incubation, seedlings were removed from the wells, homogenized, and serial dilutions plated on 1× LB to calculate the number of CFU/plant (A) and CFU/ml liquid (B) L. monocytogenes in monoculture (m) or coculture (c). We distinguished between ES620 and L. monocytogenes by color and morphology. Statistics were performed using Kruskal-Wallis ANOVA and Mann-Whitney t tests comparing coculture to monoculture CFU. Asterisks denote P values (*, P < 0.05; **, P < 0.01; ***, P < 0.001).