Figure 2.

HDL (high-density lipoprotein) composition during the postprandial response. A, Postprandial plasma samples were obtained from 20 healthy individuals at 8, hourly time points (INTERMET [acute effects of interesterification of commercially used fats on postprandial fat metabolism] study). Plasma PCSK9 (proprotein convertase subtilisin/kexin type 9) levels were measured, alongside triglycerides (TGs). B, A linear regression analysis between the PCSK9 and TG postprandial response is represented. C, Postprandial plasma samples from a further 20 healthy individuals at 8, hourly time points (validation cohort) were assessed for PCSK9 and HDL-TG content. D, Nuclear magnetic resonance–based lipoprotein analysis was conducted over the postprandial time course, and the particle concentration of S.HDL (small HDL), M.HDL (medium HDL), L.HDL (large HDL), and XL.HDL (extra-large HDL) is shown. E, Label-free proteomics was conducted upon HDL immuno-isolated from postprandial plasma samples (n=8, 3 time points), and significantly changing proteins over 8 h are represented as a heat map and protein clusters are shown graphically. Significance was determined using the nonparametric Friedman test with Dunn correction, *P<0.05, **P<0.005, ***P<0.0005, ****P<0.0001.