TABLE 2.
Oligonucleotides used in this study
| Target and primer | Sequence (5′ to 3′) |
|---|---|
| ku70 | |
| ku70-F-F | ATGGCACCCTATGATGACTGGA |
| ku70-F-R | TCAGGCGTGGTTGTCCAGC |
| ophph cassette | |
| gpd-F | TCCAAAGCCGCTCTCATGG |
| ter-R | TGCTCTATGTCTTGCCTTGTCTCG |
| 5′-flanking region of ura3 sgRNA1 site | |
| ura3-hru-1-F | CGAATGGCGGTTCTGGTCC |
| ura3-hru-1-R | CCAAGGGGCAGACCGACAG |
| 3′-flanking region of ura3 sgRNA1 site | |
| ura3-hrd-1-F | CAGGGGGCTTCTGCTCCTC |
| ura3-hrd-1-R | GCGGTGAAGGTATGTCCCTAGGA |
| 3′-flanking region of ura3 sgRNA2 site | |
| ura3-hrd-2-F | CATACACGGAAGAGGCCGTCC |
| ura3-hrd-2-R | GCGGTGAAGGTATGTCCCTAGGA |
| ku70 fragment outside ku70-sgRNA1 target | |
| ku70-1-F | AGAAAGCCACTTTTCGGCAGTGAG |
| ku70-1-R | TCCTCAGTCTCCGCCTCCCG |
| ku70 fragment outside ku70-sgRNA2 target | |
| ku70-2-F | GCATTCAGTTGACCGCATGGTACA |
| ku70-2-R | CGGAAAGGAACGACCACCTCG |
| ura3 | |
| ura3-F | ATGGTGGCCGTGGCCAA |
| ura3-R | CTAATCCGAGATCCCAACCCTTT |
| ura3 and flanking regions | |
| ura3-hru-500-F | AGGGGTGTCCTTGGAAGAAAC |
| ura3-hrd-500-R | GGCACTTATACAACCTCATTCAGAC |
| ura3 and two homologous arms | |
| ura3-HR-F | CGAATGGCGGTTCTGGTCC |
| ura3-HR-R | GCGGTGAAGGTATGTCCCTAGGA |