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GCs inhibit the transcription of the endogenous K14 keratin gene. The levels of K14 mRNA were measured by using Northern blot analysis during keratinocyte treatment with DEX. The K14 mRNA levels significantly decreased during the 12-h treatment and decreased further during 24- and 48-h treatments. RA also decreases K14 mRNA levels, as shown previously (59). In contrast, HPRT mRNA, which was used as a control, was not significantly changed during the treatments.
