TABLE 1.
Strains used in this study
| Strain | Description | Source |
|---|---|---|
| W3110 | Wild-type E. coli; F− λ− | ATCC 27325 |
| W3110/pCas | Harboring pCas in W3110 for gene editing | This study |
| WJW02 | Derived from W3110 by disrupting 25 genes (gmhD-waaQ, wbbL-rmlB) relevant to LPS polysaccharide biosynthesis | This study |
| WJW08 | Derived from W3110 by disrupting 119 genes relevant to biosynthesis of LPS polysaccharide (25 genes; gmhD-waaQ, wbbL-rmlB), EPS (28 genes; galF-wza and yccC-ymcD), flagella (57 genes; flhE-motA, fliY-fliR, and flgN-flgL), and fimbria (9 genes; fimB-fimH) | This study |
| WJW09 | Derived from W3110 by disrupting 21 genes (galF-wza) relevant to colanic acid and 7 genes (yccC-ymcD) for type IV EPS biosynthesis | This study |
| WJW010 | Derived from W3110 by disrupting 57 genes (flhE-motA, fliY-fliR, and flgN-flg) relevant to flagellum biosynthesis | This study |
| WJW011 | Derived from W3110 by disrupting 9 genes (fimB-fimH) relevant to type I fimbria biosynthesis | This study |
| W3110/pBHR68 | Harboring plasmid pBHR68 in W3110 | This study |
| WJW02/pBHR68 | Harboring plasmid pBHR68 in WJW02 | This study |
| WJW08/pBHR68 | Harboring plasmid pBHR68 in WJW08 | This study |
| WJW09/pBHR68 | Harboring plasmid pBHR68 in WJW09 | This study |
| WJW010/pBHR68 | Harboring plasmid pBHR68 in WJW010 | This study |
| WJW011/pBHR68 | Harboring plasmid pBHR68 in WJW011 | This study |