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Conversion of 2-methyl-2-cyclohexen-1-one (1a) to 2-methylcyclohexanone (1b) with the NMN+ or NADP+-based regeneration system using wild-type or engineered 6PGDH. (A) Schematic of the synthetic enzymatic pathway. (B) Profiles of production of 2-methylcyclohexanone (1b) based on regeneration system of NMNH or NADPH. Average yields of three independent trials as determined by GC relative to the internal standard are shown.
