Fig. 5. (A) Schematic description of the radiochemical complexation of apo-D5 with 67Ga. 67Ga-complexation proceeds in 5 min, 25 ∘C, pH 7. (B) A representative radiolableing HPLC trace for the characterization of 67Galbofloxacin (tR = 7.13 min, left axis, 67Ga counts per second), in comparison with HPLC characterization of the apo-D5 ligand (tR = 7.28 min, right axis, absorbance at 280 nm) is shown. Free 67Ga elutes at 0.7 minutes. (C) Time-dependent, radiochemical bacterial uptake studies in S. aureus of 67Galbofloxacin in iron deficient, DP-treated media show that uptake is comparable with 67Ga-citrate, which acts as a staphyloferrin (natural siderophore) mimic for S. aureus (n = 5). (D) On pre-incubation with 200× Fe-LDFC, the uptake of 67Galbofloxacin in S. aureus is attenuated significantly after 10 min (n = 5).
