KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, Peptides, and Recombinant Proteins | ||
| TRIzol Reagent | Invitrogen | Cat#15596026 |
| dNTPs (Set, 100mM) | Sigma-Aldrich | Cat#DNTP100 |
| DNase, RNase-free | Thermo Scientific | Cat#EN0521 |
| SYBR Green I (10 000x in DMSO) | Invitrogen | Cat#S7563 |
| Q5 High Fidelity DNA Polymerase | New England Biolabs | Cat#M04915 |
| Critical Commercial Assays | ||
| AmpliCap-Max™ T7 High Yield Message Maker Kit | Biozym | Cat#150472 |
| RevertAid First Strand cDNA Synthesis Kit | Thermo Scientific | Cat#K1621 |
| Deposited Data | ||
| kcnh8 sequences | this paper | https://github.com/dukecomeback/swordtail/blob/main/kcnh8_Xiphophorus.fa |
| RNA-seq short read sequences | [16] and this paper | ENA accession code PRJEB8012 and PRJNA673149 |
| Experimental Models: Organisms/Strains | ||
| Xiphophorus hellerii, Rio Lancetilla strain | Biocenter Würzburg | WLC1337 |
| Xiphophorus maculatus, Rio Jamapa strain Jp163A | Biocenter Würzburg | WLC6628 |
| Xiphophorus montezumae, Tamasops strain | Biocenter Würzburg | WLC1052 |
| Xiphophorus monticolus, El Tejon strain | Biocenter Würzburg | WLC3344 |
| Xiphophorus pygmaeus, strain Rio Axtla | Biocenter Würzburg | WLC3015 |
| Priapella lacandonae | Biocenter Würzburg | WLC5281 |
| Oryzias latipes, Carbio strain | Biocenter Würzburg | WLC2674 |
| Oligonucleotides | ||
| Oligonucleotide Primers for PCR | see Table S3 for primers | |
| Recombinant DNA | ||
| pcDNA3 | Thermo Fisher | V79020 |
| pNBI16/pNB1u | [66] | n.a. |
| Software and Algorithms | ||
| GSNAP version 2018-03-25 | [67] | http://research-pub.gene.com/gmap/ |
| STAR | [66] | https://github.com/alexdobin/STAR/releases |
| DESeq2 | [68] | https://bioconductor.org/packages/release/bioc/html/DESeq2.html |
| Ensembl Biomart | [69] | http://www.ensembl.org/biomart/martview/a61c56ebb44f17c0e39ce71a4d79f44a |
| DAVID | [70] | https://david.ncifcrf.gov/ |
| Inparanoid | [71] | http://inparanoid.sbc.su.se/cgi-bin/index.cgi |
| Pal2Nal | [72] | http://www.bork.embl.de/pal2nal/ |
| MUSCLE | [73] | https://www.ebi.ac.uk/Tools/msa/muscle/ |
| Gblocks | [74] | http://molevol.cmima.csic.es/castresana/Gblocks_server.html |
| Phylip | [75] | http://evolution.genetics.washington.edu/phylip.html, |
| FigTree | see website | http://tree.bio.ed.ac.uk/software/figtree/ |
| ETE3 | [76] | http://etetoolkit.org/ |
| Primer3 (version 4.4.0) | see website | https://bioinfo.ut.ee/primer3/ |
| R/qtl v.1.39-5 | see website | https://rqtl.org/ |
| diamond v0.9.24.125 | [77] | https://github.com/bbuchfink/diamond |
| CAP3 | [78] | http://doua.prabi.fr/software/cap3 |
| GeneWise wise2.2.3-rc7 | [79] | http://www.ebi.ac.uk/Wise2/ |