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. Author manuscript; available in PMC: 2021 Nov 10.
Published in final edited form as: Cell Rep. 2021 Oct 12;37(2):109815. doi: 10.1016/j.celrep.2021.109815

Figure 7. Knockdown of individual components of the MSL complex has different effects on DNA damage-induced apoptosis.

Figure 7.

(A and B) The expression of individual components of the MSL complex in HCT116 cells left untreated or treated with 40 μM cisplatin for 8 h as measured by (A) qRT-PCR and (B) immunoblots (nuclear lysates). Only the full-length mRNA isoform expression is shown for MSL1 in (A). TBP, loading control. Similar immunoblot results were obtained with total cell lysates.

(C and D) The results of apoptosis assay (40 μM cisplatin) in HCT116 cells with siRNA-mediated knockdown of individual components of the MSL complex from 2 separate sets of 3 independent experiments. The dashed line indicates the percentage of apoptotic cells from the control siRNA group with cisplatin treatment. In all panels, error bars indicate SEM and statistical significance is determined by one-tailed t test. *p < 0.05; **p<0.01.