Figure 10.

GluN2B-containing NMDA receptors mediate increased BLA activity during social investigation in adults. In isolated and non-isolated conditions for adults, BLA activity was increased in partner investigation regions compared with object (A), but there was no difference in proximal regions (B). In the ifenprodil group (n = 8), BLA activity was higher in partner investigation sites compared with an object, but the effect of isolation on BLA activity during social investigation was mitigated (C). Consistent with the vehicle group, there were no differences in BLA activity in proximal regions (D). In the vehicle adult group (n = 6), there were no differences in events (peaks three SDs above the mean) that occurred in investigation or proximal regions, while isolation increased the proportion of events in partner investigation and proximal regions when compared with object (E). In the ifenprodil group, there were no differences in events in proximity or investigation areas and isolation no longer had an effect (F). While ifenprodil had no significant effect on BLA activity preference for social investigation, ifenprodil decreased the effect of isolation on this BLA activity preference (G) but had no effect on BLA activity when in proximal areas (H). Error bars represent the SEM. Horizontal blue lines indicate the average dF/F while in the center; **p < 0.01, ***p < 0.001, post hoc two-way RM-ANOVA.