Fig. 2. Decreased frequency of DC subsets is common across respiratory infections compared to healthy donors.

Previously frozen PBMCs isolated from each group of the cohort were stained using four high-parameter flow cytometry panels. Manual gating was used to estimate the frequencies of (A) the monocyte family (fig. S1A), (B) the DC family (fig. S1A), (C) the NK cell family (fig. S1B), and (D) the T cell family (fig. S1C) for each group of the cohort. All data include at least nine patients per group (Table 1) and are represented as means ± SD. Depending on the distribution of our data, statistical analyses were performed using Kruskal-Wallis test with Dunn’s multiple comparisons test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. ns, not significant.