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. 2021 Nov 10;7(46):eabj9846. doi: 10.1126/sciadv.abj9846

Fig. 5. FGF signaling determines the CM fate by promoting Wnt signaling.

Fig. 5.

(A) Violin plots showed down-regulation of Wnt response genes Lef1 and Axin2 in the FgfrΔRet mutant transcriptome. (B) In mosaic analysis, FgfrΔRet mutant cells acquired ectopic expression of Otx2 and Mitf at the expense of Lef1. In contrast, the remaining wild-type cells identified by the lack of Otx2 and Mitf expression still maintained Lef1 expression. Scale bar, 50 μm. (C) Constitutive activation of Wnt signaling by deleting the β-cateninSer45Ser45) motif transformed βcatCA retinae to the CM as indicated by Cdo expression. Further deletion of FGFRs in FgfrΔRetcatCA mutant retinae converts them to the RPE as indicated by ectopic expression of Pcad and Otx2 as well as the appearance of pigmentation (arrows). Both the level of Lef1 and the amount of β-catenin detected by the β-catenin C-terminal antibody (βCTD) arose in βcatCA retinae but declined in FgfrΔRetcatCA mutants, showing that the Wnt–β-catenin signaling is dependent on FGF signaling. The mutant regions are marked by dotted lines. Scale bar, 100 μm.