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. 2021 Sep 29;7(5):687–700.

Table S6. Temperature conditions for real-time PCR array experiment.

Program Cycles Duration Temp.
Pre-incubation 1 10 min 95°C
Denaturation 45 15 s 95°C
Amplification 45 1 min 60°C
Melting curve analysis 1 5 s 95°C
Melting curve analysis 1 1 min 65°C
Melting curve analysis 1 - 95°C