Figure 6.

Immunofluorescence of G3BP1 to observe stress granule assembly in the presence and absence of nsp1 over 2 h of oxidative stress. HEK cells were transfected with pCAGGS-nsp1-Myc expression plasmid. Twenty-four hours later, sodium arsenite–mediated stress was induced for (top) 30, (middle) 60, and (bottom) 120 min. Cells were fixed and stained with anti-G3BP1 and anti-Myc antibodies. The image was collected using Leica TCS SP8 confocal microscope using 40× objective. The white arrow shows stress granules, and the yellow arrow shows diffused localization of G3BP1. G3BP1, Ras GTPase-activating protein SH3 domain–binding protein 1; HEK, human embryonic kidney; nsp1, nonstructural protein 1.