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. 2021 Sep 16;2(6):648–665. doi: 10.1158/2643-3230.BCD-20-0208

Figure 5.

Figure 5.

CD93 and CD123 are expressed on normal myeloid and endothelial cells. A, IHC using CD93 antibody on a tissue microarray with a panel of normal tissues (magnification equivalent to 20×; insets highlight endothelial cell staining). UMAP projection of single cells, colored either by patient sample (B) or Leiden gene expression cluster (C). D, UMAP of single cells, colored by expression of hematopoietic marker genes: PTPRC (CD45) is expressed primarily within clusters 0, 2, 4, 6, 9, 12, and 15; CD14 is expressed primarily within clusters 0, 1, 5, 6, and 8; and CD3D is expressed in cluster 2. E, UMAP projection of single cells, colored by expression of endothelial marker genes marking different endothelial subsets: CLDN5 is expressed in all endothelial clusters (1, 5, and 13); VWF is expressed in stalk-like endothelial cells (cluster 1); EDNRB is expressed in a cluster that contains tip-like endothelial cells (cluster 5); and CCL21 is expressed only in a cluster that represents endothelial venules (cluster 13). F, UMAP of single cells, colored by expression of CD93 and IL3RA (CD123) demonstrated expression in endothelial subclusters (most highly expressed in cluster 1). G, Violin plots displaying the expression level of tissue-specific markers representative of each cluster.