Figure 6.

Inhibitory effect of MCC950 on senescence phenotype. Mouse chondrocytes were stimulated with 5 ng/ml IL-1β for 24 h after pretreatment with MCC950 for 2 h. (a, b) Western blotting results and quantification analysis of senescence marker protein P16 (INK4A). (c) β-Galactosidase staining of chondrocytes. Scale bar: 100 μm. (d) Quantification analysis of β-galactosidase activity. Data are shown as the mean ± SD. Significant differences between groups are indicated as ^#P < 0.05 vs. control group; ^∗P < 0.05 and ^∗∗P < 0.01 vs. IL-1β group.