Table 1.

Protein and polysaccharide derivates hydrogels crosslinking related properties characterization.

Hydrogels source	Measurement method	Crosslinking related property	References
Polysaccharide: alginate	Compressive test	Stiffness (elastic modulus)	[20]
Polysaccharide: alginate Protein/polysaccharides collagen/tyramine hyaluronan	Gravimetric analysis	Degradation	[18, 19]
Polysaccharide: alginate	DNA content Metabolic assay test	Cell proliferation	[17–21]
Proteins and polysaccharides: alginate, collagens, fibrin, hyaluronic acid, keratin, and laminin	Atomic force microscopy AFM	Stiffness (elastic modulus) Microstructure	[18, 21, 22]
Protein/polysaccharides collagen/tyramine hyaluronan Fibrin/alginate	Rheological measurements	Storage G′ and loss G″ moduli	[19, 22]
Polysaccharide: inulin Protein/polysaccharides collagen/tyramine hyaluronan	Differential scanning calorimetry DSC	Glass transition Protein denaturation	[19, 23]
Polysaccharides: alginate; inulin Protein: fibrin Protein/polysaccharides collagen/tyramine hyaluronan	Scanning electron microscopy (SEM)	Porous diameter; morphology	[18, 19, 23]
Polysaccharides: inulin Protein/polysaccharides collagen/tyramine hyaluronan	Gravimetric analysis	Swelling ratio	[19, 23]