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. Author manuscript; available in PMC: 2021 Nov 18.
Published in final edited form as: Acc Chem Res. 2021 May 5;54(10):2409–2420. doi: 10.1021/acs.accounts.1c00056

Figure 6. Nano-scale organization of PKA activity by AKAP clustering.

Figure 6.

(A) We have used a new, superresolution PKA activity reporter FLINC-AKAR to reveal that PKA activity is organized into discrete clusters along the plasma membrane, which likely correspond to clusters of AKAP-assembled molecular signalosomes. (B) A superresolution image of a representative HeLa cell expressing FLINC-AKAR, illustrating clear activity hotspots following PKA stimulation (magnified in inset). Scale bar, 10 μm (inset, 1 μm).