A) Viable cell counts were measured in whole units of fresh-frozen plasma after thaw and centrifugation from n=10 independent donors. B) Size and granularity profile of ungated viable and non-viable cells from a representative blood and fresh-frozen plasma co-culture wells. C) Representative sample of leukocyte cells from fresh-frozen plasma was investigated for lymphocyte subpopulations using flow cytometry to evaluate size ( FSC), granularity ( SSC), and expression of lineage surface markers including CD3, CD4, CD8, CD45RO, CCR7, CD127 and CD25.