Figure 4. Viable CD3+ T cells from frozen plasma divide after co-culture with unrelated PBMCs.

A) CD3+ cells from three independent fresh-frozen plasma units (effectors) were assayed for division after culture for five days with irradiated PBMCs from unrelated donors in duplicate wells (targets; n=2 experiments). B) The frequency of division of viable CD3+ T cells was measured in wells with frozen plasma alone or co-cultured with targets from 2 experiments (Donors A through F) was analysed by paired T test. C) CD3+ cells isolated from whole blood, fresh plasma, and fresh-frozen plasma ( effectors) were assayed for division after culture for seven days in duplicate wells with irradiated PBMCs from unrelated donors ( n=2 experiments; Donors G, H).