Figure 5.

LOTUS enhanced axonal extension of the transplanted cells and serotonergic neuronal regeneration in the injured spinal cord

(A) Representative images of immunostaining for STEM121 at the sagittal sections in the control group.

(B–D) Enlarged images of the area in the white box in (A); rostral in (B), epicenter in (C), and caudal in (D).

(E) Representative images of immunostaining for STEM121 at the sagittal sections in the LOTUS group.

(F–H) Enlarged images of the area in the white box in (E); rostral in (F), epicenter in (G), and caudal in (H).

(I), Quantitative analysis of the STEM121-positive area (LOTUS group; n = 5, control group; n = 5).

(J) Representative images of immunostaining for NF-H at mid-sagittal sections at 4 mm caudal to the epicenter.

(K) Quantitative analysis of NF-H-positive area (LOTUS group; n = 5, control group; n = 5, PBS group; n = 5).

(L) Representative images of immunostaining for 5-HT at the axial sections at 4 mm caudal to the epicenter.

(M) Quantitative analysis of 5-Ht-positive area (LOTUS group; n = 5, control group; n = 5, PBS group; n = 5).

Scale bars, 1,000 μm in (A and E), 100 μm in (B–D and F–H), and 50 μm in (J and L). Values are the mean ± SEM; ^∗p < 0.05, ^∗∗p < 0.01. Statistical analysis was performed using Mann-Whitney U test in (I) and one-way ANOVA followed by the Tukey-Kramer test in (K and M).