Figure 3.

Loss of TBX3 enhances pancreatic progenitor generation from PSCs

(A) Schematic of directed differentiation protocol of PSCs to immature β cells.

(B) Representative example of PDX1 and NKX6.1 expression in PP2 cells by flow cytometry.

(C) Quantification of the percentage of PDX1⁺/NKX6.1⁺ and PDX1⁺ PP2 cells (n = 6 per cell line).

(D) Time course of PDX1, NKX6.1, ISL1, and MNX1 expression during pancreatic differentiation by qRT-PCR (n = 6 per time point, per cell line).

(E) Time course of AFP, TTR, ApoA2, and ApoB expression during pancreatic differentiation by qRT-PCR (n = 6 per time, point per cell line).

For all statistical analysis, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, and ^∗∗∗∗p ≤ 0.0001.