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. 2021 Nov 11;7:348. doi: 10.1038/s41420-021-00738-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A The expression profile of different isoforms of Axdnd1 transcripts in various mouse tissues is analyzed by RT-qPCR. B Expression of the most enriched full-length Axdnd1 transcript (Axdnd1-211) in the testes of mice aged 1, 2, 3, 4, 6, and 8 weeks. C Relative mRNA levels of Axdnd1-211 in isolated mouse testicular germ cells. D The schematic of the generation of a mouse model expressing FLAG-tagged AXDND1 is shown. E–G Western blot analyses of AXDND1 expression in multiple tissues (b), developmental testes (F), and isolated germ cells (G) from Axdnd1^FLAG/FLAG male mice are shown. GAPDH served as a loading control.