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. 2021 Nov 10;12(11):1069. doi: 10.1038/s41419-021-04059-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Analysis of the cell surface area via immunofluorescence staining after Ang II-infused hiPSC-CMs transfected with pcDNA3.1(+) or circ-SIRT1-oe or circ-SIRT1-oe plus treatment with 3-MA (autophagy inhibitor). Scale bar, 10 μm. N = 3. B, C The expressions of CH markers in Ang II-infused hiPSC-CMs of the above-mentioned groups were determined by RT-qPCR and western blot analyses. N = 3. D, E Autophagy was measured via western blot analysis of autophagy-related proteins and immunofluorescence staining analysis of Ad-GFP-mRFP-LC3 in Ang II-infused hiPSC-CMs of the above-mentioned groups. Representative images of fluorescent GFP dots (green), mRFP dots (red), and their merged images were shown. Scale bar, 10 μm. N = 3. ^**P < 0.01 was assessed by one-way ANOVA and Tukey.