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. 2021 Nov 10;7:346. doi: 10.1038/s41420-021-00746-z

Fig. 1. Moderate-intensity exercise downregulated P2X7, promoted autophagy, and inhibited pyroptosis.

Fig. 1

We set the following five groups: saline, MIA, MIA + low-intensity exercise, MIA + moderate-intensity exercise, MIA + high-intensity exercise. A total of 50 rats were randomly assigned, with 10 rats in each group. The animal tissues in this part, such as paraffin sections and joint cavity lavage fluid, came from previous experiments of our research group [23]. A TUNEL assays were used to evaluate cell death in tissue sections from each group. Red: dead cells; blue: nuclei stained by DAPI (scale bar: 50 μm). B IHC was used to evaluate P2X7, NLRP3, caspase-1, mTOR, AMPK, LC3B, and Beclin-1 expression in tissue sections from each group. Brown: stained cells; blue: hematoxylin-stained nuclei (scale bar: 500 μm). C ELISA were used to detect IL-1β content in the articular cavity lavage fluid from each group. D Statistical data for cell death in TUNEL assays. E Statistical data for stained cells in IHC analysis. The * in the histochemical images represents the positively stained cells. Data are presented as means ± standard deviations of at least three independent experiments. *p < 0.05, **p < 0.01.