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. 2021 Nov 10;7:346. doi: 10.1038/s41420-021-00746-z

Fig. 3. Supplement to Fig. 2.

Fig. 3

A TUNEL assays were used to detect cell death in tissue sections from each group. Red: dead cells; blue: nuclei stained by DAPI (scale bar: 50 μm). B IHC was used to detect the expression levels of P2X7, NLRP3, caspase-1, mTOR, AMPK, LC3B, and Beclin-1 in tissue sections from each group. Brown: stained cells; blue: hematoxylin-stained nuclei (scale bar: 500 μm). C Statistical data for cell death in TUNEL assays. D Statistical data for stained cells in IHC analyses. The * in the histochemical images represents the positively stained cells. Data are presented as means ± standard deviations of at least three independent experiments. *p < 0.05, **p < 0.01.