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. 2021 Nov 10;7:346. doi: 10.1038/s41420-021-00746-z

Fig. 6. Moderate activation of P2X7 reduced pyroptosis through AMPK/mTOR-induced autophagy.

Fig. 6

A CCK-8 assays were used to evaluate cell viability in each group. Absorbance was measured at a wavelength of 450 nm. B LDH release assays were used to detect the degree of cell damage. C Flow cytometry was used to detect the number and ratio of caspase-1/PI-stained cells, reflecting the severity of cell pyroptosis. D Statistical data for stained cells. E ELISA was used to detect IL-1β content in cell culture supernatants for each group. F Cell fluorescence experiments were used to detect the fluorescence intensity and puncta number of cell LC3B staining, reflecting the level of autophagy (scale bar: 10 μm). G Statistical data for LC3B puncta/cell. H, I Western blotting and J RT-qPCR were used to detect protein and mRNA expression levels of P2X7, NLRP3, caspase-1, mTOR, AMPK, LC3B, Beclin-1, MMP13, and collagen II. Data are presented as means ± standard deviations of at least three independent experiments. *p < 0.05, **p < 0.01.