Figure 2.

Assessment of autophagy in FKRP KO myotubes

(A) Representative images show immunostaining for MHC (green) and LC3B (in red) in WT counterpart and FKRP KO PS cell–derived myotubes at basal conditions or upon treatment with 100 μM chloroquine (CQ) DAPI-stained nuclei (in blue). Scale bar, 50 μm.

(B) Graph shows quantification of LC3B puncta (A) normalized to MHC area. Inset shows ×5 magnification. ^∗∗p < 0.01 by one-way ANOVA followed by Sidak's multiple comparison test. Each symbol represents a different field of view for four independent experiments. Error bars represent mean ± SEM.