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. 2021 Oct 14;16(11):2752–2767. doi: 10.1016/j.stemcr.2021.09.009

Figure 3.

Figure 3

Assessment of autophagy in LGMDR9 and WWS patient-specific myotubes

(A) Representative images show immunostaining for MHC (green) and LC3B (in red) in WT and LGMDR9 iPS cell–derived myotubes at basal conditions or upon treatment with 100μM chloroquine. (CQ) DAPI-stained nuclei (in blue). Scale bar, 50 μm.

(B) Graph shows quantification of LC3B puncta (A) normalized to MHC area. Each symbol represents a different field of view for two independent experiments using three WT and three LGMDR9 cell lines. ∗∗p < 0.01 by one-way ANOVA followed by Sidak's multiple comparison test. Error bars represent mean ± SEM.

(C) Representative images show immunostaining for MHC (green) and LC3B (in red) in WT1, WWS, and WWS corrected iPS cell–derived myotubes at basal conditions or treated with 100 μM chloroquine (CQ). DAPI-stained nuclei (in blue). Scale bar, 50 μm.

(D) Graph shows quantification of LC3B puncta (B) normalized to MHC area. ∗p < 0.05 by one-way ANOVA followed by Sidak's multiple comparison test. Each symbol represents a different field of view for four independent experiments. Error bars represent mean ± SEM.