Skip to main content
. 2021 Oct 14;16(11):2752–2767. doi: 10.1016/j.stemcr.2021.09.009

Figure 6.

Figure 6

TUNEL assay in FKRP mutant myotubes

(A) Representative images show immunostaining for MHC (green) and TUNEL (red) for WT, FKRP KO, LGMDR9, LGMDR9 compound heterozygous (comp. het.) WWS, and WWS corrected myotubes. DAPI stains nuclei (in blue). Scale bar, 100 μm.

(B) Graph shows quantification of TUNEL signal per MHC area for FKRP KO and parental control. Each symbol represents a different field of view for three independent experiments.∗∗p < 0.01 by unpaired Student's t test. Error bars are mean ± SEM.

(C) Graph shows quantification of TUNEL signal per MHC area for LGMDR9 and WT. Each symbol represents a different field of view for three independent experiments using three WT and three LGMDR9 cell lines. ∗∗∗∗p < 0.0001 by unpaired Student's t test. Error bars are mean ± SEM.

(D) Graph shows quantification of TUNEL signal per MHC area for LGMDR9 comp. het and WT. Each symbol represents a different field of view for three independent experiments of two WT and two LGMDR9 comp. het. lines, ∗∗∗∗p < 0.0001 by unpaired Student's t test. Error bars are mean ± SEM.

(E) Graph shows quantification of TUNEL signal of WT, WWS, and WWS corrected (E) per MHC area. ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 by one-way ANOVA followed by Sidak's multiple comparison test. Each symbol represents a different field of view for three independent experiments. Error bars are mean ± SEM.