Figure 3.

Modulation of WNT signaling affects the sensitivity of neurons to mitochondrial toxins

(A) Diagram presenting the mESC differentiation conditions.

(B and C) (B) Analysis of TH in relation to DAPI in cultures differentiated according the 5-stage protocol and (C) the medial FP protocol.

(D) Graph showing the percentage of TH-expressing neurons in relation to DAPI. Mean values ± SD; two-way ANOVA with Bonferroni correction; n = 3 independent experiments.

(E and F) (E) IHC analysis of cultures differentiated according the 5-stage protocol and (F) the medial FP protocol. Arrows point to cCASP3⁺ TH⁺-labeled neurons.

(G and H) (G) IHC analysis of cultures differentiated according the 5-stage protocol and (H) the medial FP protocol. Arrows point to cCASP3⁺ SOX6⁺-labeled neurons. Arrowheads indicate OTX2⁺ neurons negatively labeled for cCASP3.

(I–K) Graphs showing (I) the percentage of cCASP3 in relation to TH, (J) the percentage cCASP3-expressing cells in relation to SOX6, and (K) the percentage cCASP3-expressing cells in relation to OTX2 under various culture conditions. Mean values ± SD; two-way ANOVA with Bonferroni correction; n = 3 independent experiments.

^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. Scale bars, 50 μm.