Figure 5.

Induction of DA-subpopulation-specific markers in dopaminergic cultures transduced with L-SOX6 and L-OTX2

(A) IHC analysis of coronal sections of the human embryonic midbrain. Arrows indicate OTX2⁺ DA neurons, while arrowheads point to SOX6⁺ DA neurons. Higher-magnification images are from the regions indicated by a dashed square.

(B) Schematic overview of differentiation conditions. Arrow indicates time point of lentiviral transduction.

(C) IHC analysis of day-35 lentiviral transduced cultures.

(D) IHC analysis of lentivirus transduced cultures (day 35). Arrowheads indicate overlap between CALB1 and OTX2.

(E) IHC analysis of lentivirus transduced cultures at day 35. Arrowheads indicate overlap between GIRK2 and SOX6.

(F) Graph showing percentage of TH⁺ neurons expressing indicated markers at day 35. Mean ± SD; one-way ANOVA with Bonferroni correction; n = 3 independent experiments.

^∗p < 0.05, ^∗∗p < 0.01. Scale bars, 100 μm.