Fig. 3.

Efficacy of slow freezing and vitrification with conventional open and closed devices. a Primordial follicle density(/mm³). b The percentage of γH2AX-positive primordial follicles. c The percentage of AC3-positive primordial follicles. d Non-apoptotic primordial follicle density(/mm³). e Primary follicle density(/mm³). f The percentage of γH2AX-positive primary follicles. g The percentage of AC3-positive primary follicles. h Non-apoptotic of primordial follicle density(/mm³). Data are presented as mean ± SE or %. One-way ANOVA with LSD post hoc analysis was used for multiple group comparisons. VF-open, vitrification using open device; VF-closed, vitrification using the closed device; Pdf, primordial follicle; Pyf, primary follicle. *p < 0.05, compared to fresh; **p < 0.01, compared to fresh