Table 2.
Comparison of redox parameters in PCOS and control women after subgrouping as insulin resistant (IR) and non-insulin resistant (NIR)
| IR | NIR | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Redox parameters | PCOS (n = 35) | Controls (n = 12) | Pa | PCOS (n = 15) | Controls (n = 59) | Pb | Pc | Pd | Pe |
| Catalase activity (U/mL) | 4.44 (0.99, 6.62) | 6.53 (3.02, 9.45) | 0.070 | 3.94 (2.07, 6.72) | 4.49 (3.02, 8.85) | 0.50 | 0.219 | 0.470 | 0.465 |
| TBARS (MDA μM) | 7.72 (4.12, 12.79) | 4.58 (2.55, 7.05) | 0.044 | 4.91 (1.41, 9.76) | 2.88 (1.55, 4.27) | 0.048 | 0.009 | 0.460 | 0.041 |
| GR activity (U/mL) | 0.006 (0.005, 0.008) | 0.008 (0.005, 0.012) | 0.046 | 0.004 (0.003, 0.006) | 0.008 (0.006, 0.01) | 0.001 | 0.002 | 0.804 | 0.028 |
| PON1 arylesterase activity (KU/L) | 23.90 (17.19, 34.39) | 30.95 (27.68, 51.84) | 0.038 | 20.98 (9.46, 41.37) | 32.84 (19.34, 46.89) | 0.046 | 0.028 | 0.830 | 0.715 |
| SOD activity (% inhibition) | 62.41 (54.64, 68.53) | 69.66 (60.82, 72.5) | 0.049 | 49.59 (44.56, 72.08) | 66.73 (61.26, 72.97) | 0.025 | 0.041 | 0.824 | 0.989 |
| GPX activity (U/ml) | 60.32 (32.17, 88.47) | 82.44 (46.58, 141.1) | 0.049 | 80.43 (69.03, 95.84) | 91.62 (79.87, 118.0) | 0.055 | 0.045 | 0.174 | 0.022 |
| Total thiols (μM) | 168.10 (100.00, 198.60) | 198.60 (130.40, 239.10) | 0.045 | 192.8 (123.2, 218.1) | 208.7 (175.4, 253.6) | 0.049 | 0.010 | 0.715 | 0.166 |
| TAC (mM of trolox eqval) | 0.62 (0.44, 0.85) | 0.97 (0.58, 1.41) | 0.031 | 0.69 (0.43, 1.00) | 0.92 (0.61, 1.32) | 0.046 | 0.042 | 0.558 | 0.162 |
| Total glutathione | 12.97 (10.43, 16.61) | 14.63 (10.78, 19.67) | 0.450 | 15.38 (9.62, 20.14) | 15.68 (12.27, 19.47) | 0.490 | 0.755 | 0.416 | 0.990 |
| GSSG (μM) | 5.93 (3.00, 7.13) | 3.99 (2.53, 4.80) | 0.040 | 3.57 (2.24, 5.84) | 2.07 (0.86, 4.04) | 0.027 | 0.035 | 0.855 | 0.08 |
| GSH (μM) | 7.63 (4.07, 9.50) | 10.02 (8.29, 11.88) | 0.048 | 8.35 (4.66, 9.64) | 9.40 (7.82, 11.32) | 0.029 | 0.033 | 0.978 | 0.085 |
| GSH/GSSG | 2.07 (1.13, 2.96) | 4.95 (1.61, 9.92) | 0.023 | 2.39 (1.65, 3.46) | 3.79 (2.24, 7.7) | 0.040 | 0.008 | 0.805 | 0.198 |
| AOPP (μM) | 29.22 (25.93, 31.72 | 27.84 (18.27, 29.02) | 0.047 | 28.41 (26.23, 30.30) | 24.72 (20.41, 28.06) | 0.044 | 0.007 | 0.337 | 0.585 |
Data represented as median (interquartile range) for redox parameters measured in follicular fluid, compared between different subgroups. Statistical comparison was performed using Mann-Whitney U test. P < 0.05 considered significant. TBARS thiobarbituric acid reactive substances, GR glutathione reductase, SOD superoxide dismutase, GPX glutathione peroxidase, TAC total antioxidant capacity, GSSG oxidized glutathione, GSH reduced glutathione, AOPP advanced oxidized protein products, IR insulin resistant, NIR non-insulin resistant
P values obtained for different comparisons:
Pa = IR PCOS (n = 35) vs. IR controls (n = 12)
Pb = NIR PCOS (n = 15) vs. NIR controls (n = 59)
Pc = All PCOS (n = 50) vs. All controls (n = 71)
Pd = IR controls (n = 12) vs. NIR controls (n = 59)
Pe = IR PCOS (n = 35) vs. NIR PCOS (n = 15)