Table 3.
Comparison of redox parameters in PCOS and control women after subgrouping as lean and obese
| Lean | Obese | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Redox parameters | PCOS (n = 18) | Controls (n = 45) | Pf | PCOS (n = 31) | Controls (n = 26) | Pg | Pc | Ph | Pi |
| Catalase activity (U/ml) | 4.207 (2.255–7.568) | 4.662 (0.785–8.511) | 0.893 | 4.370 (1.32–6.70) | 1.321 (1.32–9.60) | 0.624 | 0.219 | 0.885 | 0.772 |
| TBARS (MDA μM) | 4.862 (1.429–9.864) | 3.879 (2.270–7.704) | 0.323 | 7.83 (4.63–12.84) | 3.97 (2.00–5.54) | 0.002 | 0.009 | 0.530 | 0.045 |
| GR activity (U/mL) | 0.004 (0.003–0.006) | 0.008 (0.006–0.011) | 0.0002 | 0.006 (0.005–0.009) | 0.009 (0.007–0.011) | 0.036 | 0.002 | 0.870 | 0.004 |
| PON1 arylesterase activity (KU/L) | 22.01 (9.28–39.37) | 32.67 (19.99–46.69) | 0.025 | 22.61 (15.77–33.61) | 32.32 (19.26–47.01) | 0.036 | 0.028 | 0.565 | 0.558 |
| SOD activity (% inhibition) | 61.18 (50.11–71.59) | 68.34 (62.36–73.41) | 0.037 | 62.22 (51.03–69.28) | 68.05 (61.12–72.55) | 0.042 | 0.041 | 0.849 | 0.476 |
| GPX activity (U/mL) | 84.45 (50.94–94.67) | 88.14 (62.00–118.0) | 0.221 | 44.24 (29.16–77.41) | 72.39 (40.21–103.2) | 0.044 | 0.045 | 0.266 | 0.0004 |
| Total thiols (μM) | 185.5 (134.1–223.9) | 215.9 (176.1–256.5) | 0.042 | 185.5 (106.5–210.1) | 198.6 (162.3–238.0) | 0.048 | 0.010 | 0.955 | 0.569 |
| TAC (mM of trolox eqval) | 0.6411 (0.435–0.946) | 0.92 (0.526–1.385) | 0.049 | 0.559 (0.458–0.936) | 0.776 (0.383–1.232) | 0.464 | 0.042 | 0.245 | 0.281 |
| Total glutathione | 18.59 (11.46–21.16) | 16.50 (14.33–21.16) | 0.877 | 12.97 (9.911–16.75) | 14.02 (10.23–16.40) | 0.961 | 0.755 | 0.529 | 0.023 |
| GSSG (μM) | 4.293 (3.385– 5.520) | 2.550 (1.782–4.607) | 0.043 | 3.649 (2.290–4.796) | 1.924 (1.045–3.765) | 0.028 | 0.035 | 0.313 | 0.710 |
| GSH (μM) | 9.065 (3.993–11.72) | 9.741 (8.497–11.78) | 0.371 | 6.785 (4.448–9.496) | 9.151 (7.238–11.53) | 0.040 | 0.033 | 0.845 | 0.009 |
| GSH/GSSG | 2.644 (1.667–3.614) | 4.096 (2.326–8.153) | 0.036 | 1.792 (1.203–2.946) | 4.772 (2.205–8.693) | 0.004 | 0.008 | 0.220 | 0.131 |
| AOPP (μM) | 27.29 (25.79–29.50) | 24.32 (20.13–28.16) | 0.040 | 29.26 (25.28–31.55) | 26.91 (20.05–29.30) | 0.038 | 0.007 | 0.264 | 0.057 |
Data represented as median (interquartile range) for redox parameters measured in follicular fluid, compared between different subgroups. Statistical comparison was performed using Mann-Whitney U test. P < 0.05 considered significant. TBARS Thiobarbituric acid reactive substances, GR glutathione reductase, SOD superoxide dismutase, GPX glutathione peroxidase, TAC total antioxidant capacity, GSSG oxidized glutathione, GSH reduced glutathione, AOPP advanced oxidized protein products
P values obtained for different comparisons:
Pf = lean PCOS (n = 18) vs. lean controls (n = 45)
Pg = obese PCOS (n = 32) vs. obese controls (n = 26)
Pc = all PCOS (n = 50) vs. all controls (n = 71)
Ph = lean controls (n = 45) vs. obese controls (n = 26)
Pi = lean PCOS (n = 18) vs. obese PCOS (n = 32)