Table 1.
TSGA10 primers used for PCR
| Target | Forward | Reverse |
|---|---|---|
| Primers used for co-segregation analysis | ||
| exon15: c.1108-1G > T | TGCTTATTTGGGTCATTTGCC | ACCTGTCGTTCTGAGTATACCA |
| Primers used for real-time PCR | ||
| Exon6 | AAGTCCAAGACGCCCATCAC | ACTGTGCCATCAAAGAGACCTC |
| Exon7 | TGGCAGAAATTCAGGGTAATG | TTGCAGGTTTAAGCAGTCTCAT |
| Exon8 | ATTACCCGACTTCGACG | CTTTCCAACCTTTAGCCTC |
| Exon9 | AGAAGGCTCACCTGGAA | CCATTGAGTGTTTATAGCCA |
| Exon10 | TCCTGTCAGTCTTGCCAT | CTTTTTGTCTGCCAAGTTC |
| Exon11 | CTTTCTGATACTCAGCGACACC | GTTAAGGGCTTGTTTCCAAGTT |
| Exon12 | GGAGAAGTTCAAAAAGTACGATA | GCCAGATCATTGAGGGTTC |
| Exon13 | ATCATTGCTGAGATGGAACA | CGTATCATTAAGCCCCTTG |
| Exon14 | CTTGTGAGAATTTGTTGCTCTG | CTTTCCCTGGCGATCTGG |
| Exon15 | TGGAGGTTAACAAGCTGAAGAA | CTTTGATTCACCTGTCGTTCTG |
| Exon16 | CAGGAATCTGAGAACCG | CAGCAGTGATAAGTTCCAG |
| Exon17 | AGGTCTTACAAGTCCCAG | CAAGTTTAATACAGAGTTCCC |
| Exon18 | TGAACTCCTGAGGAGTCAGATG | TTTTCTGCCAAACAAAGGTG |
| Exon19 | GAGTAGAGATGTGGCCCAG | AGAACTAAAACCTCTTGTTACCTA |
| Exon20 | GCCGTACAAGAACTTCGC | GATGGTGAGCACGTTCTG |
| Exon21 | CTTACGTTTTGCTGAAGTGATACAC | TTGACCTTTCTCAGGGATGTG |
| Primers used to confirm the breakpoint | ||
| Breakpoint | ACTCAGGTGATGATTTTATGGG | ACCCTCTAACTTGCTCTGCCC |
| Primers used for constructing the minigene vectors | ||
| Exon14 | CTATAGGGAGACCCAAGCTTACAGTGTACTGAGGCCCTAA | GAAAACACATAAGCCCAATAGCATTCCATTTAAAAATTATGTGGCCTA |
| Exon15 | TAGGCCACATAATTTTTAAATGGAATGCTATTGGGCTTATGTGTTTTC | GAAAACAGGTCATCCTGTAATTAACATCCAGGCATCTTTCTGAACA |
| Exon16 | TGTTCAGAAAGATGCCTGGATGTTAATTACAGGATGACCTGTTTTC | ACGTCATATGGATAGGATCCTTGCTCAACCTCTCTGTTG |
| Primers used for site directed mutagenesis | ||
| Mu1108-1 | TTTTTTCGTATGCACTGTCCAAAAAATTGAATGACACTC | TGGACAGTGCATACGAAAAAAAGATAGGTATATTTGACTATG |
| Primers used for detecting splicing isoform | ||
| Exon14-16 | ACAGTGTACTGAGGCCCTAA | TTGCTCAACCTCTCTGTTG |