Table 2.
Antibodies used in Western blot and immunofluorescence
| Primary/secondary antibodies | Host species | Company | Cat/Lot no | Application | Dilution |
|---|---|---|---|---|---|
| TSGA10 Antibody | Rabbit | Proteintech | 12,593–1-AP | WB | 1:2000 |
| IF | 1:200 | ||||
| Acetyl-α-Tubulin Rabbit mAb | Rabbit | ABclonal | 5335 T | WB | 1:2000 |
| IF | 1:1000 | ||||
| Alexa Fluor® 488—Conjugated Goat anti-Mouse IgG(H + L) | Goat | ThermoFisher | 1,834,802 | IF | 1:400 |
| Alexa Fluor® 555—Conjugated Goat anti-Rabbit IgG(H + L) | Goat | ThermoFisher | 1,774,719 | IF | 1:400 |
| Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP | Goat | ThermoFisher | 31,460 | WB | 1:5000 |
| Goat anti-Mouse IgG (H + L) Secondary Antibody, HRP | Goat | Thermofisher | 31,430 | WB | 1:5000 |