Figure 1.

Hematopoiesis-specific Cited2 deletion does not derail multilineage hematopoiesis

(A) Cited2 mRNA levels in cells isolated from 8-week-old C57BL/6 mice (n = 4).

(B) Cited2 expression in different hematopoietic compartments determined using single-cell SMART2-seq.

(C) Cited2 expression in HSCs and committed progenitor cell compartments determined by 10× Genomics single-cell RNA-seq.

(D) Cited2^fl/fl mice were bred to Vav-iCre mice to generate Cited2^fl/fl;Vav-iCre (Cited2^CKO) mice. Cited2^fl/fl mice were used as controls (Cited2^CTL).

(E) Cited2 expression in c-Kit⁺ cells from BM of Cited2^CTL and Cited2^CKO mice (n = 4).

(F) PB counts of 8- to 10-week-old mice (n = 14).

(G) Total BM cellularity (n = 6–9).

(H) Total number of differentiated myeloid (Gr-1⁺Mac-1⁺) and B cells (CD19⁺B220⁺) in BM and spleen (Spl) (n = 6–9).

(I) Total number of mature CD4⁺ and CD8⁺ T cells in spleens (n = 6–9).

(J) Total number of Lin⁻ cells, LK cells, pre-GM (LKCD41⁻FcγRII/III⁻CD150⁻CD105⁻), GMP (LKCD41⁻FcγRII/III⁺), Pre-MegE (LKCD41⁻FcγRII/III⁻CD150⁻CD105⁺), MkP (LKCD41⁺CD150⁺) cells (n = 5).

(K) Total number of Pre-CFU-E (LKCD41⁻FcγRII/III⁻CD150⁺CD105⁺), CFU-E (LKCD41⁻FcγRII/III⁻CD150⁺CD105⁺CD71⁺Ter119⁻), and Pro Ery (LKCD41⁻FcγRII/III⁻CD150⁺CD105⁺CD71⁺Ter119⁺) cells (n = 5).

(L) Colony-forming unit (CFU) assay performed with 10⁴ BM cells from 8- to 10-week-old mice. CFU-megakaryocyte (CFU-Mk), CFU-erythroid (CFU-E), burst-forming unit (BFU-E), CFU-granulocyte (CFU-G), CFU-monocyte/macrophage (CFU-M), CFU-granulocyte, and monocyte/macrophage (CFU-GM); at least three lineages (CFU-Mix), CFU with all four lineages, granulocyte, erythroid, monocyte/macrophage, and megakaryocyte (CFU-GEMM) (n = 7).

(M) Colony counts of primary and secondary cultures (n = 7). For (B), (C), and (F)–(M), data are mean ± SEM. ^∗ p < 0.05, ^∗∗p < 0.01