Figure 2.

Cited2 is required for the maintenance of the HSC pool and its expansion upon aging

(A) Total number of LSK cells, LSKCD48⁻CD150⁺Flt3⁻CD34⁻ HSCs, LSKCD48⁻CD150⁺Flt3⁻CD34⁺ MPP1, LSKCD48⁺CD150⁺Flt3⁻ MPP2, LSKCD48⁺CD150⁻Flt3⁻ MPP3, and LSKCD48⁺CD150⁻Flt3⁺ MPP4 populations in BM of 8- to 10-week-old mice (n = 6–9).

(B) Total number of LSK cells, HSCs, MPP1, MPP2, MPP3/MPP4, and LK cells in BM in 52-week-old mice (n = 6).

(C) BM cellularity in 52-week-old mice (n = 6–9).

(D) Total number of differentiated myeloid cells (Gr-1⁺Mac-1⁺) and B cells (CD19⁺B220⁺) in BM, spleen (Spl) and PB in 52-week-old mice (n = 6–9).

(E) PB counts in 52-week-old Cited2^CTL and Cited2^CKO mice (n = 6–9). For (A)–(E), data are mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01.