Figure 3.

HSCs critically require Cited2 to reconstitute hematopoiesis upon transplantation

(A) Transplantation assay: 100 HSCs or 2,000 LSK cells sorted from Cited2^CTL and Cited2^CKO mice were transplanted into lethally irradiated recipients together with 200,000 support CD45.1⁺ total BM cells.

(B and C) Percentage of donor-derived CD45.2⁺ cells in (B) PB and (C) the LSK cell compartment of recipient mice following transplantation of 100 HSCs (n = 5 recipients per donor; 2 donors per genotype).

(D and E) Percentage of donor-derived CD45.2⁺ cells in (D) PB and (E) the LSK compartment of the recipient mice following transplantation of 2,000 LSK cells (n = 4 recipients per donor; 4 donors per genotype).

(F) Homing assay: 160,000 LSK cells were injected into irradiated CD45.1⁺-recipient mice and analyzed 18 h later.

(G) Percentage of donor-derived CD45.2⁺ cells in BM (n = 4–5).

(H) Percentage of HSCs from 8- to 10-week-old Cited2^CKO and Cited2^CTL mice in the G₀ (DAPI⁻Ki67⁻), G₁ (DAPI⁻Ki67⁺), and S/G₂/M (DAPI⁺Ki67⁺) phases of the cell cycle (n = 3).

(I) Percentage of Annexin V⁺ cells in the HSC, MPP, HPC-1, and HPC-2 cell compartments in BM of Cited2^CKO and Cited2^CTL mice (n = 3). For (B)–(E) and (G)–(I), data are mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001.