Figure 4.

Mcl1 partially restores normal HSC maintenance and function of Cited2-deficient HSCs

(A) Expression of Cited2, Mcl1, Pten, Gata1, Gata2, Foxo3a, and Bmi1 in LSK cells sorted from 8-week-old Cited2^CTL and Cited2^CKO mice (n = 4).

(B) Schematic representation of experimental mouse cohorts; Cited2^CTL, Cited2^CTL;Mcl1, Cited2^CKO, and Cited2^CKO;Mcl1.

(C) Total number of HSC, MPP1, MPP2, and MPP3/MPP4 cell populations in BM of 8- to 10-week-old mice (n = 4–6 mice per genotype).

(D) Transplantation assay: 100 HSCs were transplanted into lethally irradiated recipients together with 200,000 unfractionated CD45.1⁺ BM cells.

(E) Percentage of donor-derived CD45.2⁺ cells in PB following transplantation (n = 4 recipients per donor; n = 2–3 donors).

(F) Percentage of donor-derived CD45.2⁺ cells overall in the monocyte, granulocyte, B cell, and T cell compartments of PB.

(G) Percentage of donor-derived CD45.2⁺ cells in total BM and HSC compartments of the recipient mice.

(H) CFU assay performed with 10⁴ BM cells from 8- to 10-week-old mice (n = 5).

(I) Total CFC counts of primary and secondary cultures (n = 5). For (A), (C), and (E)–(I), data are mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01.