FIG. 2.

Defective endocrine cell differentiation and islet morphogenesis in hnf6^−/− mice. In hnf6^−/− mice, the number of glucagon (glu)-expressing cells was normal at e10.75 (A and B) but was reduced at e12.5, e15.5, and P4 (C to H). However, the number of clusters of early glucagon-expressing cells was normal at e12.5 and e15.5 (arrows in panels C to F). In hnf6^−/− mice, there was a reduced number of insulin (ins)-expressing cells at e15.5 and P4 (I to L), of somatostatin (som)-expressing cells at P4 (M and N), and of PP-expressing cells at P4 (O and P). At P4, hormone-producing cells were found near pancreatic ducts in hnf6^−/− mice, instead of being organized in islets as in control littermates (G, H, and K to P). Original magnifications: ×400 (A to D, I, and J) and ×312.5 (E to H and K to P).