Figure 4.

Gal-9 decreases the release of cyto-chemokines in knee joints. Inflammatory fluids obtained from knee joint homogenates at 18h time-point, were assayed using a proteome profiler cytokine array for (A) CTRL, (B) MSU and (C) MSU + Gal-9 group. Densitometric analysis is presented as heatmap (D). Thereafter, IL-10, IL-17, KC, JE, MIP-1α and TNF-α were extrapolated from heatmap and represented graphically (E). Data (expressed as INT/mm²) are presented as means ± SEM. of positive spots of three separate independent experiments run each with n = 6 mice per group pooled. Statistical analysis was performed by using two-way ANOVA followed by Bonferroni’s test for multiple comparisons. ^###P ≤ 0.001, ^####P ≤ 0.0001 vs Ctrl group; *P ≤ 0.05, **P ≤ 0.01, ****P ≤ 0.0001 vs MSU group.