Skip to main content
. 2021 Oct 28;9:757378. doi: 10.3389/fbioe.2021.757378

FIGURE 1.

FIGURE 1

Representative full scan and regions of interest (ROIs) from patients and ROI-based correlation matrices on 84-plex RNA and 40-plex proteins. (A) Representative full-scan image for patient 1. (B) Representative ROI images for all patients. For every patient, 12 ROIs were drawn, and four representative ROIs (2 tumors and 2 stroma) are shown with tricolor fluorescence labeling (blue: SYTO13, green: Pan-cytokeratin, Pan-CK, red: CD45) at 20x magnification (patients 1 and 2: chemotherapy only, patients 3 and 4: chemotherapy + immunotherapy). For correlation matrices, ROIs are grouped based on treatment options, ROI location (tumor or stroma), and patient IDs. (C) mRNA-based expression correlation matrix shows separate stroma and tumor ROI clusters, and separate therapy-dependent ROI clusters in tumor regions. QC-failed and normal epithelium ROIs were excluded. (D) Protein-based expression correlation matrix shows separate stroma and tumor ROI clusters. QC-failed and normal epithelium ROIs were excluded.