FIG. 4.

mutRFXANK does not bind to RFXAP. (A) Schematic representation comparing mutRFXANK from BLS (Bequit and Nacera) with the wild-type RFXANK protein. A 26-bp deletion at the boundary between intron 5 and exon 6 in the RFXANK gene directs the synthesis of a protein that contains 124 residues, of which 90 are from RFXANK, followed by 34 irrelevant residues and a premature stop codon (tga). The white portion of the mutRFXANK depicts the first 90 residues that are unchanged, ending in LPATLD. The frameshift starts a new sequence, colored in gray. Residues from position 91 on (WCRPPH…) are not present in the wild-type protein. (B) mutRFXANK does not bind to RFXAP in vitro. GST pull-down assays were performed with three different GST fusion proteins [GST-RFXANK, GST-RFXANK(1-90) and GST-mutRFXANK]. Results are presented in lanes 1 to 4; lane 5 contains 25% of the input labeled RFXAP (i); 25% of the input GST-fusion proteins are also presented in the bottom panel.