FIGURE 4.

Sulfa‐4/22 in inhibit pyroptosis in BV2 cells, and the binding validation with GSDMD. (A‐C) Effects of Sulfa‐4/22 on the expression of key pyroptosis proteins (n = 3): NLRP3 and Caspase‐1 were relatively high in the DMSO group. Sulfa‐4/22 could inhibit NLRP3 and Caspase‐1. In the DMSO group, GSDMD was relatively high, and p30‐GSDMD was relatively low. Comparison with the DMSO group, *p < 0.05. (D) Validation of the binding between GSDMD and Sulfa‐4 by Co‐IP: Sulfa‐4 could directly bind to GSDMD, which had no binding relationship with Caspase‐1. (E‐F) Validation of the binding between GSDMD and Sulfa‐4 by pull‐down assay: Sulfa‐4 could directly bind to GSDMD