FIGURE 5.

Validation of the targeted binding relationship between Sulfa4/22 and GSDMD. (A) Competitive assay showed that the Biotin‐labelled Sulfa‐4 could be replaced by the unlabelled Sulfa‐4, indicating that the two sites were both GSDMD. (B) Validation of the binding between GSDMD and Sulfa‐22 by Co‐IP: Sulfa‐22 could directly bind to GSDMD, which had no binding with Caspase‐1. (C‐D) Validation of the binding between GSDMD and Sulfa‐22 by pull‐down assay: Sulfa‐22 could directly bind with GSDMD. (E) Competitive assay revealed that Biotin‐labelled Sulfa‐22 could be replaced by unlabelled Sulfa‐22, indicating that the two sites were both GSDMD